Introduction:

EMSA (also known as gel-shift assay) can be used to study the binding of target proteins to specific DNA sequences, thereby investigating the activation levels of certain transcription factors within cells. The EMSA/Gel-Shift Sample Loading Buffer (Blue, 4×) is suitable for sample loading after the binding reaction between nuclear proteins or purified transcription factors and specific double-stranded oligonucleotides in EMSA studies.

The EMSA/Gel-Shift Sample Loading Buffer (Blue, 4×) contains bromophenol blue, which may sometimes interfere with the binding reaction between the target transcription factor and specific double-stranded oligonucleotides. If interference occurs, the bromophenol blue-free EMSA/Gel-Shift Sample Loading Buffer (Colorless, 4×) is available as an alternative.

Storage: Store at -20℃ for 12 months. It is recommended to freeze and store in aliquots to avoid repeated freezing and thawing.

Protocol (for reference only):

Mix the sample with the loading buffer at a ratio of 3 μL sample to 1 μL Loading Buffer (4×). After mixing thoroughly, load the sample immediately.

Notes:

1. Other reagents required for EMSA experiments need to be prepared by the user.
2. For professional research use only. Not for use in clinical diagnosis or treatment, not for food or pharmaceuticals, and must not be stored in ordinary residential areas.
3. For your safety and health, please wear personal protective equipment and clothing when operating.