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Service Introduction

    Escherichia coli expression system is the earliest and most widely used classical expression system in gene expression technology. Compared with other expression systems, E. coli expression system has the characteristics of clear genetic background, high expression level of target gene, short culture period and strong anti-pollution ability. It occupies an important position in gene expression technology and is an important tool in the process of molecular biology research and biotechnology industrialization.

 

Customer provides

    Recombinant expression plasmid, quality >1μg

    Target gene sequence

    Vector sequence and map

 

Final delivery product

    1-3 mg of purified target protein in 2 L fermentation broth

    Experiment report

 

Ordering Information

    Project

    Project Description

    Lead time

    Tag fusion protein expression and purification(E.coli

    One-step affinity column purification, purity >80%

    1-2 weeks

    Affinity column + more than two purification steps, >90% purity

    2-3 weeks

    Expression and purification of non-affinity-tagged proteins

    Customized Purification Protocols

    3-4 weeks

 

Additional service options

    Project

    Project Description

    Lead time

    total gene synthesis

 

    2-3 weeks

    subclone

    Optional vectors pGEX series, pET series, pSUMO

    1 week

    Inclusion body renaturation

 

    1-2 weeks

    Label Removal

 

    1-2 weeks

 

Inquiry form download
Mail: service@wshtbio.com
4006-021-390