Introduction：

Conventional Tris-SDS PAGE Gels can only resolve normal molecular weight proteins, and the resolution is extremely low for proteins with small molecular weights, especially proteins with molecular weight below 10kD. Tricine-SDS PAGE Gels provide separation of low molecular weight proteins and peptides. Tricine-SDS Gel System is a modification of the traditional tris-glycine gel system that uses a discontinuous buffer system specifically designed for the resolution of low molecular weight proteins.

Tricine-SDS PAGE Gel Preparation Kit includes a full set of reagents required for the preparation of Tricine-SDS PAGE gels. You only need to prepare a small amount of distilled water to prepare the high-quality gels of various concentrations according to the instructions. After electrophoresis, the gels can be directly used for gel staining, silver staining, Western blot hybridization and other experiments.

Product Contents：

Storage：

1. The shelf life is three months if the kit stored at 4℃ in dark.
2. PAGE Gel Coagulant should be stored at -20℃. If the coagulant is stored at 4°C, the shelf life is three months.

Protocol：

Please select the appropriate separating gel concentration according to the molecular weight of the target protein.

Gel concentration formula reference table:

Note: Volumes of stacking gel, intermediate gel and separating gel differ according to the thickness of gel casting.