| Specification number | Specification | market price | member price | quantity | add to Shopping Cart |
|---|---|---|---|---|---|
| EZWB16-7 | 50T | $70.00 |
|
Introduction：
UARE-TBE PAGE is a denaturing polyacrylamide gel for the analysis and purification of single-stranded DNA or RNA of 20–800 bases in length. It is ideal for synthetic oligonucleotide analysis and purification, RNase protection assays, in vitro transcription studies, and Northern blotting experiments. UREA TBE PAGE Gel Preparation Kit includes a full set of reagents required for the preparation of UREA TBE PAGE gels. You only need to prepare a small amount of distilled water to prepare the high-quality gels of various concentrations according to the instructions.
Product Contents：
|
Reagent Number |
Reagent A |
Reagent B |
Reagent C |
Reagent D |
|
Reagent Name |
30%Acr |
UREA |
Gel Buffer |
PAGE Gel Coagulant |
|
Specification |
100 mL |
100 g |
50 mL |
5 mL |
|
Storage |
4℃ in the dark |
Room Temperature |
4℃ |
-20℃ |
Storage：
Protocol:
Please select the appropriate separating gel concentration according to your experimental needs.
Gel concentration formula reference table:
|
Items |
Stacking Gel |
Separating Gel |
|||
|
Gel Percentage |
4% |
8% |
10% |
12% |
15% |
|
30%Acr |
0.8 mL |
2.4 mL |
3 mL |
3.6 mL |
4.5 mL |
|
UREA |
2.52 g |
3.78 g |
3.78 g |
3.78 g |
3.78 g |
|
Gel Buffer |
1.2 mL |
1.8 mL |
1.8 mL |
1.8 mL |
1.8 mL |
|
PAGE Gel Coagulant |
60 μL |
90 μL |
90 μL |
90 μL |
90 μL |
|
ddH2O |
Make up to volume |
Make up to volume |
Make up to volume |
Make up to volume |
Make up to volume |
|
Total Volume |
6 mL |
9 mL |
9 mL |
9 mL |
9 mL |
Note: Volumes of stacking gel and separating gel differ according to the thickness of gel casting.
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