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TBE PAGE Gel Preparation Kit
Product number : EZWB16-6
describe :
Specification : 50T
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EZWB16-6 50T
$70.00

Introduction

TBE PAGE is applied to electrophoresis of nucleic acids from 50 to 2,000 base pairs. It’s an ideal tool for DNA electrophoresis applications including analysis of restriction enzymes digestion, PCR products, Southern blot and primer. TBE PAGE Gel Preparation Kit includes a full set of reagents required for the preparation of TBE PAGE gels. You only need to prepare a small amount of distilled water to prepare the high-quality gels of various concentrations according to the instructions.

 

Product Contents

Reagent Number

Reagent A

Reagent B

Reagent C

Reagent Name

30%Acr

Gel Buffer

PAGE Gel Coagulant

Specification

100 mL

50 mL

5 mL

Storage

4℃ in the dark

4℃

-20℃

 

Storage

  1. The shelf life is three months if the kit stored at 4℃ in dark.
  2. PAGE Gel Coagulant should be stored at -20. If the coagulant is stored at 4°C, the shelf life is three months.

 

Protocol:

Please select the appropriate separating gel concentration according to your experimental needs.

Gel concentration formula reference table:

Items

Stacking Gel

Separating Gel

Gel Percentage

4%

6%

8%

10%

12%

15%

30%Acr

0.8 mL

1.8 mL

2.4 mL

3 mL

3.6 mL

4.5 mL

Gel Buffer

1.2 mL

1.8 mL

1.8 mL

1.8 mL

1.8 mL

1.8 mL

ddH2O

4.0 mL

5.4 mL

4.8 mL

4.2 mL

3.6 mL

2.7 mL

PAGE Gel Coagulant

60 μL

90 μL

90 μL

90 μL

90 μL

90 μL

Total Volume

6 mL

9 mL

9 mL

9 mL

9 mL

9 mL

Note: Volumes of stacking gel, intermediate gel and separating gel differ according to the thickness of gel casting.

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