| Specification number | Specification | market price | member price | quantity | add to Shopping Cart |
|---|---|---|---|---|---|
| EZWB16-8 | 50T | $70.00 |
|
Introduction：
Electrophoretic mobility shift assay (EMSA) is an experimental technique to study the interaction of DNA-binding proteins with their associated DNA sequences. This technique is based on the fact that DNA-protein complexes migrate slower than non-bound DNA in a native polyacrylamide or agarose gel, resulting in a “shift” in migration of the labeled DNA band. EMSA PAGE Gel Preparation Kit includes a full set of reagents required for the preparation of EMSA PAGE gels. You only need to prepare a small amount of distilled water to prepare the high-quality gels of various concentrations according to the instructions.
Product Contents：
|
Reagent Number |
Reagent A |
Reagent B |
Reagent C |
Reagent D |
|
Reagent Name |
30%Acr |
Gel Buffer |
50% Glycerol |
PAGE Gel Coagulant |
|
Specification |
50 mL |
25 mL |
15 mL |
5 mL |
|
Storage |
4℃ in the dark |
4℃ |
Room Temperature |
-20℃ |
Storage：
Protocol:
Please select the appropriate separating gel concentration according to your experimental needs.
Gel concentration formula reference table:
|
Gel Percentage |
4% |
4.5% |
5% |
6% |
6.5% |
|
30%Acr |
0.8 mL |
0.9 mL |
1 mL |
1.2 mL |
1.3 mL |
|
Gel Buffer |
0.6 mL |
0.6 mL |
0.6 mL |
0.6 mL |
0.6 mL |
|
50% Glycerol |
0.3 mL |
0.3 mL |
0.3 mL |
0.3 mL |
0.3 mL |
|
ddH2O |
4.3 mL |
4.2 mL |
4.1 mL |
3.9 mL |
3.8 mL |
|
PAGE Gel Coagulant |
30 μL |
30 μL |
30 μL |
30 μL |
30 μL |
|
Total Volume |
6 mL |
6 mL |
6 mL |
6 mL |
6 mL |
Note: Volumes of stacking gel and separating gel differ according to the thickness of gel casting.
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