Introduction:

Gel Red is a highly sensitive, non-mutagenic, ultra-safe and ultra-stable fluorescent stain (at working concentrations) for visualization of nucleic acids in agarose or acrylamide gels. The electrophoresis bands are less prone to tail and disperse, and there is no need to reduce the sample load, and it is suitable for a wide range of applications. It is an alternative to ethidium bromide (EtBr, EB) with much higher sensitivity than EB without the need for destaining. Gel Red has the same spectral properties as EB, and it can replace EB without changing the imaging system.

Storage: Store at room temperature.

Protocol (for reference only):

1. Dilute the concentrated stain 1:10,000 in molten agarose gel solution. For example, if you require 50 mL of molten agarose for your tray, add 5 µL of 10000× Gel Red stain concentrate to 50 mL of molten agarose gel solution and mix well.

Note: Gel Red has excellent thermal stability, and it can be added directly to the high temperature molten agarose gel solution without waiting for the gel solution to cool before adding.

2. You can also dilute the concentrated stain 1:10,000 in agarose gel buffer (e.g., 1× TBE or 1× TAE) and add the buffer plus stain mixture to the powdered agarose. For example, if you run TBE gels and require 50 mL of molten agarose for your tray, add 5 µL of 10,000× Gel Red stain concentrate to 50 mL of 1× TBE, mix well, and add to the powdered agarose. Then heat the agarose/Gel Red stain mixture in the microwave.
3. Carry out electrophoresis according to conventional methods.

Notes:

1. The conductivity of TAE and TBE is different. If you need to shorten the electrophoresis time, you can choose TAE buffer.
2. Gel Red does not need to be refrigerated at low temperature. Please store it at room temperature to avoid precipitation. If precipitation is found, please heat the stain to 45-50°C for 2 minutes, and shake to dissolve, which will not affect the use effect.
3. Gel Red can be used to stain single-stranded DNA and RNA, but its sensitivity to single-stranded DNA or RNA is lower than that of double-stranded DNA.
4. For research use only. Not for use in diagnostic procedures.
5. For your safety and health, please wear personal protective equipment and clothing when operating.