Introduction:

UltraFast electrophoresis buffer is used in combination with traditional Tris-Glycine SDS-PAGE protein gel to achieve rapid electrophoresis. Compared with traditional running buffer, UltraFast electrophoresis buffer significantly shortens electrophoresis time and improves experiment efficiency. When electrophoresis is performed with UltraFast buffer, the time required for electrophoresis at 250V is about 20 minutes, which is about an hour faster than using conventional buffers. The whole process of electrophoresis from sample preparation to imaging can be completed within 30 minutes if UltraFast buffer is used in combination with WSHT's other products, including precast gel (GLASS Gel; Cat. #: GSG2001), fluorescence loading dye Quick-Bands (Cat. #: ES008), and fluorescence protein marker (Cat. #: MKP004).

UltraFast electrophoresis buffer currently comes in two different formulations. The powder pack contains 10 small packages, each of which can prepare 500 mL 1× buffer. The liquid pack contains two bottles of 500 mL 5× stock buffer solution, which should be diluted to 1× buffer before use.

Application: UltraFast electrophoresis buffer is used for Tris-Gly SDS-PAGE gel electrophoresis.

Product features:

1. Rapid running time.

Use an ice bath when the running buffer temperature exceeds 40°C. The recommended voltage is 200V.

2. High-resolution band separation.

3. Small amount of buffer volume required. The total buffer volume required for electrophoresis is only 500 mL, which greatly saves cost.

Storage:

UltraFast electrophoresis buffer (powder) can be stored at room temperature for 2 years.

UltraFast electrophoresis buffer (liquid) can be stored at room temperature for 1 month.

Cautions

1. Use 1× working solution within 2 weeks of preparation if not stored in a sealed container or within 1 month if properly sealed.

2. Under high voltage and temperature, air bubbles may appear in the gel. However, it will not affect electrophoresis results. Use an ice bath when the temperature exceeds 40°C.

3. For your safety and health, please wear the lab coat and disposable gloves before the experiments.

Instructions:

1. Sample preparation

To denature protein, use the fluorescence loading buffer Quick-Bands (Cat. #:ES008) and boil the mixture of protein and buffer at 100℃ or boiled water for 5-10 minutes.

2. Gel selection

2.1 Precast Gel (GSG2001)

Choose the Tris-Glycine gel (GSG2001) with appropriate concentration according to the molecular weight of protein.

2.2 Gel Preparation Kit (EZWB16-4)

Prepare the chosen type of Tris-Glycine electrophoresis gel according to the manufacturer’s instructions of Gel Preparation Kit (EZWB16-4).

3. Sample loading

Load the prepared protein samples into sample wells after it cools down to room temperature.

4. 1× buffer solution preparation

4.1 Powder: Dissolve one small package of powder with distilled water and dilute the solution to the final volume of 500 mL.

4.2 Liquid: Dilute 100 mL of 5× stock buffer solution with distilled water to the final volume of 500 mL.

5. Electrophoresis

Suggestion: Keep a constant voltage of 250V during electrophoresis and complete the electrophoresis in about 20 min.

After electrophoresis, protein sample that is prepared with fluorescence loading buffer can be observed, imaged, and analyzed directly under UV lamp or LED lamp, or with gel imaging system without the need of gel transferring, staining and destaining. The sensitivity of Quick-Bands (Cat. #:ES008) is higher than that of silver staining.

6. Summary

Using UltraFast electrophoresis buffer in combination with WSHT's other products, including precast gel (GLASS Gel; Cat. #: GSG2001), fluorescence loading dye (Quick-Bands; Cat. #:ES008), and fluorescence protein marker (Cat. #: MKP004), the whole process of electrophoresis from sample preparation to imaging can be completed within only 30 minutes.