Introduction:

MES Running buffer is formulated for separation of small- to medium-sized proteins on Bis-Tris gels. This product is available in powder form, each pack can prepare 5 L of 1× working solution.

Storage: Store at room temperature, valid for 2 years.

Protocol (for reference only):

1. Take 1 pack of powder that can prepare 5 L of MES running buffer, pour it into a clean beaker, add 400 mL deionized water and stir to dissolve the powder completely.
2. Make up to 500 mL in the graduated cylinder with deionized water, which is 10× mother liquor and should be diluted to 1× concentration before use.
3. Measure 50 mL of 10× mother liquor, pour it into a clean beaker, add deionized water to about 400 mL, and mix well.
4. Make up to 500 mL in the graduated cylinder with deionized water, which is 1× working solution and can be used after mixing.
5. Unused running buffer can be stored at room temperature, usually 2-3 days at room temperature and 1-2 weeks at 4°C.

Notes:

1. 1× working buffer should not be used for more than 2 weeks after preparation.
2. This running buffer is suitable for Bis-Tris gels, and should not be used for gels with other chemistries.
3. For research use only. Not for use in diagnostic procedures.
4. For your safety and health, please wear personal protective equipment and clothing when operating.