Introduction:

Tris-Tricine Running buffer is formulated for separation of proteins in their denatured state on tricine gels. Tricine substitutes glycine in the running buffer, resulting in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides in tris-tricine buffer system. This product is a 5× concentrated solution, which needs to be diluted to 1× working concentration before use.

Storage: Store at 4℃, valid for 1 year.

Protocol (for reference only):

1. Measure 100 mL of 5× anode running buffer, pour it into a clean beaker, add deionized water to about 400 mL, and mix well.
2. Make up to 500 mL in the graduated cylinder with deionized water, which is 1× working anode running buffer and can be used after mixing.
3. Prepare 1× cathode buffer in the same way as the anode buffer.
4. Add 1× Tris-Tricine Cathode Buffer to the inner buffer tank to cover the wells.
5. Add 1× Tris-Tricine anode buffer to the outer buffer tank of the electrophoresis device, assemble, and connect to the inner buffer tank.

Notes:

1. If the amount used each time is very small, it can be stored in aliquots.
2. For research use only. Not for use in diagnostic procedures.
3. For your safety and health, please wear personal protective equipment and clothing when operating.