Introduction:

WSHT's Mini EasyAgarose Precast Gels are safe and convenient precast agarose gels with high-performance for horizontal nucleic acid electrophoresis, which can significantly improve experimental efficiency of nucleic acid electrophoresis.

• Stable quality: Strict quality control of raw materials, automatic gel casting process, and perfect product sampling inspection system to ensure stability and repeatability.
• Convenient option: Just put the gel into the electrophoresis tank and load samples to conduct electrophoresis.
• Fast choice: Special formula enables electrophoresis to be completed in 10 minutes under 23-25 V/cm voltage.
• Efficient detection: Easy-access tray designed for direct imaging.  
• Safe analysis: Non-toxic nucleic acid dyes are pre-added.
• Complete variety: Two systems, TAE and TBE, are available in a variety of concentrations.
• Long shelf life: 6 months at 4°C.
• Package contents: box of 10 gels.
• For research use only. Not for use in diagnostic procedures.

Protocol:

1. Additional materials required for electrophoresis:

2. Remove Mini EasyAgarose Precast Gel from the package and place the gel in the electrophoresis tank along with the tray. The end with the sample wells should be placed close to the negative electrode.

3. Add 0.5×TBE (or 1×TAE) running buffer to the tank until the liquid level covers the surface of the gel.

Note: The precast agarose gels are pre-added with non-toxic nucleic acid dyes, no need to add dyes in the buffer.

4. Prepare sample: Mix DNA sample loading buffer (5×) (Cat. #: LB1) with sample in 1:4 ratio (volume).

5. Slowly add the sample mixture to the submerged gel wells by using a pipette. Load the Marker in the same way. The loading volume is approximately 30-40 μL.

Note: Do not insert the pipette tip too much into the sample wells to avoid puncturing the gel.

6. Run the gel: Turn on the power, the red electrode is the positive electrode, and the black is the negative electrode. DNA samples move from the negative electrode to the positive electrode (the end close to the sample wells is negative). The voltage is usually set to 120 V~180 V, and the electrophoresis time varies with the size of the electrophoresis tank. The larger the electrophoresis tank, the longer the electrophoresis time. If fast electrophoresis is desired, the voltage can be adjusted to 23 V/cm (for example, if the distance between the positive and negative electrode lines is 13 cm, the voltage is set to 13 cm×23 V/cm≈300 V). Determine whether to terminate electrophoresis according to the position where the indicator moves.

Note: Due to the large heat generation of TAE gel, it is necessary to appropriately reduce the voltage for electrophoresis, or perform water bath electrophoresis.

7. After electrophoresis is complete, take out the gel, and place the tray directly under the gel imaging system to observe the position of the electrophoresis bands and take pictures. The non-toxic dye contained in the gel has the same spectral characteristics as EB, and can be observed and photographed under UV and fluorescence (594 nm).

Note: The gel comes with a specially designed UV-transparent tray for easy handling and photographing of the gel.

Note: To remove the gel, insert a knife or any flat tool into the bottom of the gel and lift the gel from the U-shaped tray.