Introduction:

WSHT's GLASS Gels (TBE PAGE) are safe and convenient precast polyacrylamide gels with high-performance, which applied to electrophoresis of nucleic acids from 50 to 2,000 base pairs. It’s an ideal tool for DNA electrophoresis applications including analysis of restriction enzymes digestion, PCR fragment, Southern blot and primer.

• Automated gel casting technology can ensure stability and repeatability.
• Gel cassettes can be easily opened with the Gel Knife, simply push the Gel Knife gently through each side of the gel cassette.
• Compatible with mainstream mini electrophoresis tanks on the market, such as Bio-Rad, Tanon and Junyi Dongfang, etc.
• Providing high resolution of nucleic acids; molecules differing in size by only a single base pair can be resolved under the appropriate conditions.
• GLASS Gels (TBE PAGE) are available in different acrylamide concentrations. We have 5%、10%、15% of separating gels. Customized services for special concentrations are also available.

Product Data:

• Height of 4% stacking gel: 1.5cm
• Ratio of acrylamide to methylene bis acrylamide: 29:1
• Gel thickness: 1.5mm
• Well format: 10wells/15wells
• Max sample loading volume: 30μL/well for 15wells, 60μL/well for 10wells
• Cassette size: 98×84×4.1mm
• Gel size: 81×74×1.5mm
• Storage: stored at 4-8℃ for 1-2 months; do not freeze.
• Package contents: box of 10 gels.
• For Research Use Only. Not for use in diagnostic procedures.

Protocol：

1. Prepare sample: Mix TBE sample loading buffer (5×) (Cat.#:LB1)with sample in 1:4 ratio (volume).

2. Prepare running buffer: Mix 200mL of WSHT’s TBE Running Buffer（5×）(Cat.#: HTRB1001) with 800mL of deionized water to obtain 1L 1× TBE running buffer.

4. Take the GLASS Gel (TBE PAGE) out of the bag. Assemble the corresponding electrophoresis tank, add the running buffer, and then gently pull the comb out of the cassette.

5. Rinse the wells several times with 1× running buffer to remove storage buffer before loading samples. Load the appropriate volume of your samples in the appropriate wells.

Note: Do not insert the pipette tip too much into the sample wells to avoid sample leakage caused by deformation of the glass plates.

6. Run the gel: The electrophoresis conditions are usually 150V, 50~70 min.

Note: The actual electrophoresis time depends on the gel concentration.

7. After electrophoresis is complete, retrieve the gel from gel cassette: Inserting the Gel Knife between the two glass plates and gently push the Gel Knife through each side of the gel cassette; then open the gel cassette and gently peel away the gel from the plate.