Introduction:

TAE buffer (Tris- Acetate -EDTA Buffer) is a commonly used DNA electrophoresis buffer, commonly used in agarose gels, less used for PAGE gels. When the resolution requirement of electrophoresis is not high, both TAE and TBE can be used. When the resolution requirements are relatively high, TAE is suitable for electrophoresis of lower concentration gels to improve the resolution of large molecular weight nucleic acids, and on the contrary, TBE is used to improve the resolution of small molecular weight nucleic acid with the use of higher concentration gels.

This product is a 50× concentrated solution, which needs to be diluted to 1× or 0.5× working concentration according to the requirements of the experiment before use.

Storage: Store at 4℃ for 12 months.

Protocol (for reference only):

1. Prepare 1× TAE: Dilute 20 mL of 50× TAE running buffer with 980 mL of deionized or distilled water. Label as 1× TAE.
2. Prepare 0.5× TAE: Dilute 10 mL of 50× TAE running buffer with 990 mL of deionized or distilled water. Label as 0.5× TAE.

Notes:

1. For research use only. Not for use in diagnostic procedures.
2. For your safety and health, please wear personal protective equipment and clothing when operating.