Introduction:

TBE buffer (Tris-Borate-EDTA Buffer) is a commonly used DNA electrophoresis buffer, commonly used in PAGE gels, but also in agarose gels. When the resolution requirement of electrophoresis is not high, both TAE and TBE can be used.

When the resolution requirements are relatively high, TAE is suitable for electrophoresis of lower concentration gels to improve the resolution of large molecular weight nucleic acids, and on the contrary, TBE is used to improve the resolution of small molecular weight nucleic acid with the use of higher concentration gels.

This product is a pre-mixed powder: no need to weigh or adjust the pH value, each pack can be directly dissolved and mixed into a 500 mL 10× storage solution. It must be dissolved in distilled water or deionized water to prepare 1× or 0.5× working concentration according to specific experimental requirements before use.

Storage: Store at room temperature for 24 months.

Protocol (for reference only):

1. Prepare 10×TBE from powder

1. Fill a graduated cylinder or beaker with approximately 400 mL of distilled water and place a magnetic stir bar into the container.
2. Place the container on a stir plate and slowly add the contents of 1 package of 10× TBE to the water.
3. Mix the solution until the TBE is completely dissolved, then add distilled water to bring the final volume to 500 mL.

2. Prepare 1× TBE: Dilute 100 mL of 10× TBE with 900 mL of deionized or distilled water. Label as 1× TBE.
3. Prepare 0.5× TBE: Dilute 50 mL of 10× TBE with 950 mL of deionized or distilled water. Label as 0.5× TBE.

Notes:

1. For research use only. Not for use in diagnostic procedures.
2. For your safety and health, please wear personal protective equipment and clothing when operating.