
Still frustrated with the blocking step? 😫 😫
Blocking: 1 hour ⌛
Primary antibody: overnight ⌛
Secondary antibody: 1 hour ⌛
Washing forever... 😫
Running one WB requires 3 cups of coffee to stay awake?
Heartbroken over expensive antibodies?
WSHTBio
Presents the all-new Western No-Block Universal Antibody Diluent
Eliminates the blocking step entirely, making antibody incubation more convenient.
Speed up your experiments and cut antibody usage in half!
Why No Blocking?
"Triple Molecular Shield + Steric Hindrance Optimization"
- Blocks non-specific sites: Unique polymers precisely cover hydrophobic areas on the membrane, preventing off-target antibody binding and reducing background by 70%.
- Antibody stabilizer: Maintains antibody activity while reducing aggregation and precipitation. Antibody recovery rate > 90%.
- Zero-interference formula: Free of animal proteins, biotin, and phosphates. Compatible with HRP and AP chemiluminescence systems.
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Five Key Advantages
- No-block formula: After diluting antibodies, directly apply to the membrane after transfer. No blocking step needed — fast and convenient.
- High versatility: Can be used for both primary and secondary antibodies. Suitable for WB, IHC, ELISA, IF, and more.
- Enhanced performance: Contains multiple antibody protectors to reduce non-specific binding, improving specificity and sensitivity.
- Strong compatibility: Biotin-free, avoiding interference with biotin-based detection. Compatible with HRP, AP, and biotin-labeled secondary antibodies.
- High safety: Uses environmentally friendly preservatives for safer operation.
Three-Step Incubation
- After transfer, no blocking needed. Rinse briefly with TBST for 30 seconds.
- Mix antibody with diluent and incubate directly.
- Recover antibodies. Reusable when stored at 4°C.
Key Parameters
- Primary antibody incubation: 1 hour at room temperature or overnight at 4°C (recovery recommended at 4°C).
- Secondary antibody incubation: 30 minutes at room temperature. Reusable up to 3 times.
- Washing: TBST, 5 minutes × 3 times (same as traditional method).
Hard Data Comparison

Ordering Info
