Acrylamide is the main raw material for configuring SDS PAGE gel electrophoresis. Various animal experiments have shown that acrylamide is moderately toxic. In the process of contact with acrylamide, if you do not pay attention to the protection, it will cause irreversible bodily harm to personnel. The toxicity of acrylamide is reflected in the following four aspects:
1. neurotoxicity
Numerous animal studies have shown that acrylamide mainly causes neurotoxicity. The neurotoxic effects are mainly peripheral neurodegenerative changes and degeneration of parts of the brain involved in learning, memory and other cognitive functions.
2. reproductive developmental toxicity
Reproductive toxicity is manifested as decreased sperm count and motility, as well as morphological changes and decreased fertility.
3. genotoxicity
Acrylamide has been shown to be mutagenic in both in vivo and in vitro tests, causing gene mutations and chromosomal abnormalities in mammalian somatic and germ cells, such as micronucleus formation, sister chromatid exchange, polyploidy, aneuploidy and other Mitotic abnormalities, etc., the dominant lethal test was positive.
4. carcinogenicity
Animal studies have found that acrylamide can cause tumors in various organs in rats, including breast, thyroid, testis, adrenal gland, central nervous system, oral cavity, uterus, pituitary gland, etc. The International Agency for Research on Cancer (IARC) evaluated its carcinogenicity in 1994, and classified acrylamide as a Class 2 carcinogen (2A), that is, a possible human carcinogen. The main basis is that acrylamide can be metabolized into its carcinogenic active metabolite glycidamide in both animals and humans.
In the preparation process of SDS PAGE, the experimenter will inevitably come into contact with acrylamide. Therefore, when configuring the electrophoresis gel, you must pay attention to personal protection, wear various protective equipment, such as masks and gloves, and carry out in a fume hood. If you do not pay attention to protection during the preparation process, acrylamide can enter the human body through powder breathing, contact, etc., causing bodily harm to the experimenter.
The precast polyacrylamide gel completely eliminates the possible human toxicity of acrylamide to the experimenter. In the precast gel, acrylamide has polymerized to form polyacrylamide, which is no longer toxic, and the experimenter no longer has the opportunity to come into contact with acrylamide. Therefore, the use of precast gel can not only save time and improve experimental efficiency, but also protect the human health of experimenters.
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