Service Process

    ●Protein extraction and quantification (if protein samples are provided, electrophoresis is directly carried out for pre- experiments to detect antibody specificity);

    ●SDS-PAGE 10-15% denaturing polyacrylamide gel electrophoresis;

    ●Transfer membrane (PVDF membrane);

    ●Blocking (5% skim milk powder);

    ●Primary antibody incubation (internal reference gene is Tubulin or HistoneH3.1);

    ●Secondary antibody incubation (if the experiment cannot be continued due to material degradation or primary antibody, relevant pre-experimental fees will be charged);

    ●Chemiluminescence detection (ECL)

    ●Imaging

Service Contents

*The experiment can be completed in 2 weeks in general. In special cases, such as poor primary antibody, low protein concentration or special proteins such as large molecular weight proteins, the delivery time will be extended.